Detection of serum proteins by high-pressure gel-permeation chromatography with low-angle laser light scattering, compared with analytical ultracentrifugation.

Use of low-angle laser light scattering with gel permeation chromatography for the molecular mass determination of biomolecules.

486 Use of low-angle laser light scattering with gel permeation chromatography for the molecular mass determination of biomolecules K. Jumel,* P. Br0wne.t and J. F. Kennedy# *Chembiotech Ltd, Institute of Research and Development, Vincent Drive, Edgbaston, Birmingham B I5 2sQ, U.K.; tLDC Analytical, Diamond Way, Stone Business Park, Stone, Staffs STI 5 OHH, U.K. and $Research Laboratory for the...

Evaluation of a symmetry-based strategy for assembling protein complexes.

We evaluate a strategy for assembling proteins into large cage-like structures, based on the symmetry associated with the native protein's quaternary structure. Using a trimeric protein, KDPG aldolase, as a building block, two fusion proteins were designed that could assemble together upon mixing. The fusion proteins, designated A-(+) and A-(-), comprise the aldolase domain, a short, flexible s...

Determination of the molecular mass of new L-fucose-containing glycosaminoglycan and its distribution by high-performance gel-permeation chromatography with laser light-scattering detection.

Standardization of heparins by means of high performance liquid chromatography equipped with a low angle laser light scattering detector.

This study shows that HPLC-LALLS (high performance liquid chromatography with a light-scattering detector) is a convenient and reliable method for the characterization of standard heparin samples, provided that polyelectrolyte artefacts are suppressed by a suitable dialysis procedure. The method has been successfully applied to the assessment of calibrated relations like the Mark-Houwink relati...

The Structure and Nature of Protein-Polvsaccharide Complexes

Many food systems consist of mixtures of protein and polysaccharide and many are also thermally processed. Potential interaction phenomena in solutions ofprotein and polysaccharide are described using the following probes: sedimentation velocity and sedimentation equilibrium analytical ultracentrifugation, dynamic light scattering and total intensity light scattering coupled to an on-line gel p...